Journal: Scientific reports

Article Title: Blood-brain barrier and gut barrier dysfunction in chronic kidney disease with a focus on circulating biomarkers and tight junction proteins.

doi: 10.1038/s41598-022-08387-7

Figure Lengend Snippet: Figure 5. Tight junction protein expression in subcutaneous adipose tissue. (A) Immunofluorescence staining of tight junction proteins (red) claudin-5, occludin, and JAM-1, nuclear staining with DAPI (blue), endothelial marker CD31 (green) in subcutaneous tissue of kidney transplant recipients and donors. Bar = 100 µm. Expression of tight junction proteins (B) claudin-5, (C) occludin and (D) JAM-1 in subcutaneous tissue of kidney transplant recipients and donors. Results expressed in medians and interquartile range. Statistical significance *p < 0.05, **p < 0.01.

Article Snippet: Primary antibodies used were claudin-5 (1:200, CSB-PA005507LA01HU, Cusabio Technology, USA), occludin (1:200, CSB-PA016263LA01HU, Cusabio Technology), and JAM-1 (1:400, CSB-PA897579LA01HU, Cusabio Technology), and incubated overnight at 4 °C in 3% Triton and 5% goat serum in 1X PBS.

Techniques: Expressing, Immunofluorescence, Staining, Marker

Journal: Scientific reports

Article Title: Blood-brain barrier and gut barrier dysfunction in chronic kidney disease with a focus on circulating biomarkers and tight junction proteins.

doi: 10.1038/s41598-022-08387-7

Figure Lengend Snippet: Figure 6. TMAO incubation and tight junction protein expression in subcutaneous adipose tissue. (A) Immunofluorescence staining of tight junction proteins (red) claudin-5, occludin, and JAM-1, nuclear staining with DAPI (blue), endothelial marker CD31 (green) in subcutaneous tissue incubated with (A) TMAO and (B) control media. Bar = 100 µm. Expression of tight junction proteins (B) claudin-5, (C) occludin and (D) JAM-1 in subcutaneous tissue after incubation with TMAO and control media. Results expressed in medians and interquartile range. Statistical significance *p < 0.05, **p < 0.01.

Article Snippet: Primary antibodies used were claudin-5 (1:200, CSB-PA005507LA01HU, Cusabio Technology, USA), occludin (1:200, CSB-PA016263LA01HU, Cusabio Technology), and JAM-1 (1:400, CSB-PA897579LA01HU, Cusabio Technology), and incubated overnight at 4 °C in 3% Triton and 5% goat serum in 1X PBS.

Techniques: Incubation, Expressing, Immunofluorescence, Staining, Marker, Control

Journal: Journal of translational autoimmunity

Article Title: Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells.

doi: 10.1016/j.jtauto.2023.100202

Figure Lengend Snippet: Fig. 3. Immunofluorescent antibody staining in naïve and treated HUVECs. AK. The mixture of APS total IgG and β2GPI induces a pronounced increase of the protein levels of the proinflammatory cytokines IL-6, IL-8 as well the transcription factor NF-κB1 and cell adhesion molecules Tissue Factor, ICAM-1, VCAM-1, Eselectin, P- selectin and TGFR1. There was no significant difference for the TGFR1 molecule (3 J). Visual analysis revealed that all the inflammatory mediators and adhesion molecules presented statistically significant difference between the untreated and treated endothelial cells (3 K).

Article Snippet: Coverslips were incubated overnight at 4 ◦C with primary antibodies against IL-6 (5 μg/ ml, CSB-PA06757A0Rb, Cusabio), IL-8 (5 μg/ml, CSB-MA083271A0m, Cusabio), NF-κB1 (5 μg/ml, CSB-PA190132, Cusabio), TGF-β2 (5 μg/ ml, CSB- PA07319A0Rb, Cusabio), Tissue Factor (5 μg/ml, 4509, American Diagnostica), ICAM-1 (5 μg/ml, AF796, R&D Systems), VCAM-1 (4 μg/ml, sc-18854, Santa Cruz Biotechnology), E-selectin (4 μg/ml, sc-271267, Santa Cruz Biotechnology), P-selectin (4 μg/ml,sc137054, Santa Cruz Biotechnology) and TGFR1 (5 μg/ml,CSBPA023451LA01HU, Cusabio).

Techniques: Staining

Journal: Journal of translational autoimmunity

Article Title: Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells.

doi: 10.1016/j.jtauto.2023.100202

Figure Lengend Snippet: Fig. 5. Immunofluorescent antibody staining in placenta biopsies from APS patients and healthy women. A-K Placenta biopsies derived from APS patients as well as Healthy Donors show increased signal intensity for IL-6, IL-8, NF-κB1, ICAM1, VCAM-1, E-selectin, P-selectin, TGF-β2, and TGFR1 (5A-5D, 5F-5J). Slight difference in fluorescence intensity between HD and APS patient was observed for Tissue Factor (5E). Increased signal intensity was observed as well for the TNF-α molecule in the APS placenta biopsies (5 K).

Article Snippet: Coverslips were incubated overnight at 4 ◦C with primary antibodies against IL-6 (5 μg/ ml, CSB-PA06757A0Rb, Cusabio), IL-8 (5 μg/ml, CSB-MA083271A0m, Cusabio), NF-κB1 (5 μg/ml, CSB-PA190132, Cusabio), TGF-β2 (5 μg/ ml, CSB- PA07319A0Rb, Cusabio), Tissue Factor (5 μg/ml, 4509, American Diagnostica), ICAM-1 (5 μg/ml, AF796, R&D Systems), VCAM-1 (4 μg/ml, sc-18854, Santa Cruz Biotechnology), E-selectin (4 μg/ml, sc-271267, Santa Cruz Biotechnology), P-selectin (4 μg/ml,sc137054, Santa Cruz Biotechnology) and TGFR1 (5 μg/ml,CSBPA023451LA01HU, Cusabio).

Techniques: Staining, Derivative Assay, Fluorescence

Journal: Journal of Cancer

Article Title: PRMT7 Inhibits the Proliferation and Migration of Gastric Cancer Cells by Suppressing the PI3K/AKT Pathway via PTEN.

doi: 10.7150/jca.88102

Figure Lengend Snippet: Figure 1. PRMT7 is expressed at low levels in gastric cancer tissues. A Immunohistochemical analyses were performed to observe PRMT7 expression in gastric cancer: a) normal gastric tissue; b) high-medium differentiated gastric cancer tissue; c) poorly differentiated gastric cancer tissue. B Western blot analysis of PRMT7 protein expression levels in 30 paired gastric cancer tissues and adjacent normal mucosal tissues. N: normal mucosa adjacent to cancer; T: tumor tissue. C PRMT7 expression in the GES-1 cell line and various gastric cancer cell lines. ***P < 0.001.

Article Snippet: Sections were incubated with rabbit anti-Homo sapiens (Human) PRMT7 polyclonal antibody (CSB-PA885738LA01HU, CUSABIO, Wuhan, China) at 1:100 dilution overnight at 4°C followed by a goat anti-rabbit/mouse secondary antibody.

Techniques: Immunohistochemical staining, Expressing, Western Blot

Journal: Journal of Cancer

Article Title: PRMT7 Inhibits the Proliferation and Migration of Gastric Cancer Cells by Suppressing the PI3K/AKT Pathway via PTEN.

doi: 10.7150/jca.88102

Figure Lengend Snippet: Figure 2. PRMT7 inhibited gastric cancer cell proliferation and migration. A The knockdown and overexpression efficiency of the PRMT7 was detected by western blot and RT-qPCR. B CCK-8 assays were performed to compare experimental groups with the si-NC group, and PRMT7 downregulation promoted cell growth, while PRMT7 overexpression did not. C Colony formation experiments were performed and the number of colonies in the experimental group was compared to that in the si-NC group, and

Article Snippet: Sections were incubated with rabbit anti-Homo sapiens (Human) PRMT7 polyclonal antibody (CSB-PA885738LA01HU, CUSABIO, Wuhan, China) at 1:100 dilution overnight at 4°C followed by a goat anti-rabbit/mouse secondary antibody.

Techniques: Migration, Knockdown, Over Expression, Western Blot, Quantitative RT-PCR, CCK-8 Assay

Journal: Journal of Cancer

Article Title: PRMT7 Inhibits the Proliferation and Migration of Gastric Cancer Cells by Suppressing the PI3K/AKT Pathway via PTEN.

doi: 10.7150/jca.88102

Figure Lengend Snippet: Figure 3. Effect of PRMT7 expression on PTEN and downstream PI3K/AKT signaling pathway. Western blot was used to detect the effect of low/high PRMT7 expression on the expression of PTEN and related proteins in the downstream PI3K/AKT signaling pathway. *P < 0.05, **P < 0.01, ***P < 0.001 and ns: no significance.

Article Snippet: Sections were incubated with rabbit anti-Homo sapiens (Human) PRMT7 polyclonal antibody (CSB-PA885738LA01HU, CUSABIO, Wuhan, China) at 1:100 dilution overnight at 4°C followed by a goat anti-rabbit/mouse secondary antibody.

Techniques: Expressing, Western Blot

Journal: Journal of Cancer

Article Title: PRMT7 Inhibits the Proliferation and Migration of Gastric Cancer Cells by Suppressing the PI3K/AKT Pathway via PTEN.

doi: 10.7150/jca.88102

Figure Lengend Snippet: Figure 4. PRMT7 plays a tumor suppressor role dependent on PTEN. A Effect of PRMT7 overexpression on the viability of PTEN-deficient gastric cancer cells. B Effect of PRMT7 overexpression on the proliferation of PTEN-deficient gastric cancer cells. C Effect of PRMT7 overexpression on the migration of PTEN-deficient gastric cancer cells. D

Article Snippet: Sections were incubated with rabbit anti-Homo sapiens (Human) PRMT7 polyclonal antibody (CSB-PA885738LA01HU, CUSABIO, Wuhan, China) at 1:100 dilution overnight at 4°C followed by a goat anti-rabbit/mouse secondary antibody.

Techniques: Over Expression, Migration

Journal: Journal of Cancer

Article Title: PRMT7 Inhibits the Proliferation and Migration of Gastric Cancer Cells by Suppressing the PI3K/AKT Pathway via PTEN.

doi: 10.7150/jca.88102

Figure Lengend Snippet: Figure 5. PRMT7 affects gastric cancer cell proliferation and migration via the PI3K/AKT signaling pathway. A CCK-8 assay was used to detect the effect of LY294002 on the viability of gastric cancer cells. B Colony formation assay was used to detect the effect of LY294002 on the proliferation of gastric cancer cells. C Transwell assay was used to

Article Snippet: Sections were incubated with rabbit anti-Homo sapiens (Human) PRMT7 polyclonal antibody (CSB-PA885738LA01HU, CUSABIO, Wuhan, China) at 1:100 dilution overnight at 4°C followed by a goat anti-rabbit/mouse secondary antibody.

Techniques: Migration, CCK-8 Assay, Colony Assay, Transwell Assay

Journal: Journal of Cancer

Article Title: PRMT7 Inhibits the Proliferation and Migration of Gastric Cancer Cells by Suppressing the PI3K/AKT Pathway via PTEN.

doi: 10.7150/jca.88102

Figure Lengend Snippet: Figure 6. PRMT7 interacts with PTEN and promotes PTEN methylation. A RT-qPCR was used to detect the expression of PTEN mRNA in the transfected cells. B Endogenous PTEN and PRMT7 immunoprecipitation occurred in AGS cells. C Exogenous PTEN and PRMT7 co-immunoprecipitated in AGS cells. D AGS cells transfected with PRMT7 siRNA and overexpression plasmid were subjected to co-immunoprecipitation with PTEN antibody, followed by detection of PTEN methylation level with MMA antibody. *P < 0.05, **P < 0.01, ***P < 0.001 and ns: no significance.

Article Snippet: Sections were incubated with rabbit anti-Homo sapiens (Human) PRMT7 polyclonal antibody (CSB-PA885738LA01HU, CUSABIO, Wuhan, China) at 1:100 dilution overnight at 4°C followed by a goat anti-rabbit/mouse secondary antibody.

Techniques: Methylation, Quantitative RT-PCR, Expressing, Transfection, Immunoprecipitation, Over Expression, Plasmid Preparation